Figure 5 | Scientific Reports

Figure 5

From: Nitric oxide mediated inhibition of antigen presentation from DCs to CD4+ T cells in cancer and measurement of STAT1 nitration

Figure 5

Mass spectrum of a peptide from STAT1 containing Tyr701. Top panel – Full length STAT1 protein was treated with peroxynitrite and subjected to trypsin cleavage. The resulting peptides were run on the HPLC and Orbitrap mass spectrometer. The HPLC and mass spectra of the native peptide are presented. Bottom panel - Spectrum of NO-treated STAT1 (GTGnYIK). There is a shift in the retention time and m/z ratio observed for nitrated Tyr701. The fragmentation pattern important for identifying the nitration moiety is demonstrated in the figure. Labeling for N-terminal or “b” fragments and C-terminal or “y” fragments is consistent with standardized nomenclature in proteomics research. Please refer to55 for more details on this nomenclature.

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