Figure 2 | Scientific Reports

Figure 2

From: Extremely low frequency pulsed electromagnetic fields cause antioxidative defense mechanisms in human osteoblasts via induction of •O2 and H2O2

Figure 2

Effect of single and repetitive exposures to ELF-PEMF on ROS formation in hOBs. (a) Comparison of the ROS level in hOBs (N = 6, n = 4) after single exposure to ELF-PEMF (black line) and stimulation with different concentrations (0, 0.0015625, 0.003125, 0.00625, 0.0125 and 0.025%) of H2O2. (b) ROS levels in hOBs (N = 12, n = 4) after single and repetitive exposures to ELF-PEMF (7 min per day). ROS levels were determined using the DCFH-DA assay. Results are given as fold of unstimulated hOBs (Ø). *p < 0.05 and ***p < 0.001 as compared to corresponding unstimulated hOBs (two-tailed Mann-Whitney test, α = 0.05).

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