Figure 2
From: Human ATG3 binding to lipid bilayers: role of lipid geometry, and electric charge
ATG3 and ATG3 K9D/K11D insertion into lipid monolayers. (A) Representative time courses of adsorption of ATG3 (left) or ATG3 K9D/K11D (right) at an air-water interface. (B) Representative time courses of increase in lateral pressure after ATG3 (0.2 μM) addition to PC:DOPE:CL (60:30:10 mol ratio) or PC:DOPE:PA (50:30:20 mol ratio) monolayers (left). Representative time courses of increase in lateral pressure after ATG3 or ATG3 K9D/K11D addition to PC:DOPE:CL (60:30:10 mol ratio) monolayers (right). (C) Maximum increase in lateral pressure after ATG3 (left) or ATG3 K9D/K11D (right) addition to lipid monolayers. Lipids were: [⚫] PC:DOPE (70:30 mol ratio), [▲] PC:DOPE:CL (60:30:10 mol ratio), [▫] PC:DOPE:PA (50:30:20 mol ratio). Data reported as a function of initial lateral pressure.
