Figure 2
Specific detection in pure samples and demonstration of mixed bacterial imprinted film. (a)–(d) Scanning electron microscope (SEM) images of 4 types of bacteria. (e) The results of specific detection of E. coli O157:H7 with the imprinted film of E. coli O157:H7 in pure samples. Detection time was 300 s. Inset; the time dependence of QCM frequency for 4 types of bacteria including E. coli. O-157:H7 (pink line). Black line indicates the result with no bacteria, and other lines indicate the results for S. enterica, V. parahaemolyticus, and S. aureus. The concentration of bacteria was 1 × 108 CFU/mL. (f) Specific detection of complementary 4 types of bacteria with mixed bacterial imprinted film in pure samples. 2 types of mismatched bacteria (E. coli. O26: H11, B. subtilis) were also dispersed in the sample droplet. Detection time was 300 s. In Fig. (e), (f), the average of three time measurements was taken for each bacterium. Vertical bars indicate the standard deviation of the frequency change.
