Figure 3 | Scientific Reports

Figure 3

From: Hypoxia induces epithelial-mesenchymal transition in colorectal cancer cells through ubiquitin-specific protease 47-mediated stabilization of Snail: A potential role of Sox9

Figure 3

Attenuation of the EMT and metastatic potential by silencing of USP47 in CRC cells under hypoxic conditions. (a) Comparison of protein expression of USP47, E-cadherin (E-cad), N-cadherin (N-cad), and vimentin in siUSP47- or control siRNA (siCtrl)-transfected DLD-1 cells under hypoxic conditions. Protein expression was measured by Western blot analysis. (b) Immunocytochemical analysis of siControl- and siUSP47-transfected DLD-1, HCT-116, and HT-29 cells in hypoxia. Cells were stained for USP47, E-cad, N-cad, vimentin, and phalloidin. Scale bar = 20 μm. (c) Representative photo-micrographs depicting morphological and actin cytoskeleton modification in USP47-silenced DLD-1 cells under hypoxic conditions. Cells were stained for detection of actin. Scale bar = 20 μm. (d) Images of wound closure of after knockdown of USP47 in DLD-1 cells under hypoxic conditions. Scale bar = 100 μm. (e) USP47-silienced DLD-1 cells were subjected to trans-well invasion assays under hypoxic conditions. Scale bar = 150 μm. The statistical significance was assessed using a two-sided student’s t-tests; *P < 0.05, **P < 0.01, and # P < 0.01.

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