Figure 6 | Scientific Reports

Figure 6

From: Hypoxia induces epithelial-mesenchymal transition in colorectal cancer cells through ubiquitin-specific protease 47-mediated stabilization of Snail: A potential role of Sox9

Figure 6

Sox9-mediated upregulation of USP47 expression in hypoxia. (a,b) Effects of hypoxia on expression of Sox9 and USP47 proteins and their mRNA transcripts in CRC cells were assessed by Western blot (a) and PCR analyses (b), respectively. (c,d) Cells were subjected to PCR (c) and Western blot (d) analyses to assess the expression of USP47 in siSox9-transfected CRC cells. (e) Schematic diagram illustrating the position of putative Sox9 binding elements located in the promoter of human USP47 gene. (f) Direct binding of Sox9 to the USP47 promoter segment A. A ChIP assay was carried out using chromatin prepared from CRC cells. The binding of Sox9 to the USP47 promoter was detected by visualization of the PCR product. Results are representative of three independent experiments. Significance was assessed using two-sided student’s t-test; *P < 0.05, **P < 0.01, and ***P < 0.001.

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