Figure 1 | Scientific Reports

Figure 1

From: Passage-dependent accumulation of somatic mutations in mesenchymal stromal cells during in vitro culture revealed by whole genome sequencing

Figure 1

Characteristics of peripheral blood-derived MSCs. (A) Immunophenotype analyses of using flow cytometry. Red histograms represent antibody staining and black histograms indicate isotype matched IgG controls. (B) Gene expression of stemness markers by RT-PCR. (C) Evaluation of mesodermal differentiation potential in terms of adipogenesis (Oil red O), osteogenesis (Alizarin red) and chondrogenesis (Alcian blue and Safranin O) (×100). (D) qRT-PCR of native (d0, left) and differentiated (d14, right) cells presenting the expression of genes associated with trilineage differentiation. The results are represented as mean and standard deviation from three independent experiments.

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