Figure 3 | Scientific Reports

Figure 3

From: Passage-dependent accumulation of somatic mutations in mesenchymal stromal cells during in vitro culture revealed by whole genome sequencing

Figure 3

Abundances and spectra of somatic mutations acquired during ex vivo expansion of MSCs. (A) Mutational abundances (i.e., numbers of SNVs) are shown across P1 to P9 for the two MSC lines (MSC1 and MSC2) examined. The numbers of SNVs are shown above each bar. The SNVs were identified by comparing MSCs at each passage with their matched control cells. (B) The six categories of mutation spectra with respect to sequence change are shown as fractions (%) of the given somatic SNVs across the passages. (C) The functional categories or location of SNVs with respect to nearby genes are shown. Exonic mutations include missense (nonsynonymous), silent (synonymous), nonsilent, and splicing mutations. TSS mutations are those residing upstream or downstream of the transcription start sites.

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