Figure 6
From: Neuroprotective effect of Ruminococcus albus on oxidatively stressed SH-SY5Y cells and animals
Protective effects of CRA-CM against fragmentation of nuclei in oxidatively stressed SH-SY5Y cells. (A) Hoechst 33258 (5 μg/mL) staining was performed in SH-SY5Y cells. Apoptotic cells were identified by the condensation and fragmentation of their nuclei (red arrow). The negative control Caco-2 cells were treated with PBS. The expression levels of apoptosis-related genes and proteins (Bcl-2 and Bax) in oxidatively stressed SH-SY5Y cells were measured by qPCR (B) and western blotting and cropped blots are displayed (C), respectively. Western blot analysis was quantified using β-actin as an internal control (D). The full-length blots are shown in Supplementary Fig. S4. The data are representative of three independent experiments performed in triplicate.
