Figure 5

Inhibition of EphB6 action enhances drug-resistance of T-ALL cells in vivo. (A–E) ΔB6-Jurkat and pc3-Jurkat cells were resuspended in PBS and mixed 1:1 with Corning Matrigel Matrix. Cells (3 × 10⁶) were injected in a total volume of 100 µL subcutaneously into the right flank of NOD-SCID mice. As tumours reached a measurable size, mice were treated weekly with intravenous injections of Dox (2 mg/kg) or a matching volume of saline control (n = 7 per group). Tumour growth was monitored twice a week by measurements with digital callipers and tumour volume was calculated by the equation: A/2*B², where A was long and B was short diameter of the tumour (A). The reduction in tumour growth in Dox-treated mice is presented as a percentage relative to matching saline-treated controls (B). In panels (A and B), day 0 indicates the day, when both treatment and measurements were initiated. Upon experiment termination, tumours were extracted, fixed in 10% formalin, photographed (C) and weighed. The average tumour weight for each experimental group is shown (D). The graph in panel (E) represents average tumour weights in Dox-treated mice as a percentage relative to matching saline-treated controls. Data are shown as means ± SD. One of two independent experiments is shown. *P < 0.05; Student’s t-test. n.s., statistically not significant.