Figure 1

(a) The re-oxidation spectra of reduced WT-hFMO3 at four different time points. (b) Re-oxidation processes of WT-hFMO3 (black line) and N61S variant (blue line) followed at 450 nm over time. Formation and decay of the C4a-hydroperoxyFAD intermediate followed at 381 nm; (c) WT-hFMO3 and (d) N61S variant. The figure insets show the formation of the C4a-hydroperoxyFAD intermediate followed at 381 nm during the first five seconds.