Figure 4

DSC data for WT-hFMO3 (a) and N61S variant (b) in the absence (black line) and presence (red line) of 0.5 mM NADP⁺, upon heating at a scan rate of 30 °C/h. Deconvolution of the DSC profiles: (c) WT-hFMO3; (d) WT-hFMO3 incubated in 0.5 mM NADP⁺; (e) N61S variant; (f) N61S variant incubated in 0.5 mM NADP⁺. The original scan is shown in black with the deconvoluted curves shown in red and blue.