Table 1 Kinetic parameters of reductive and re-oxidative half-reactions calculated for hFMO3 wild type and N61S variant.

From: Inactivation mechanism of N61S mutant of human FMO3 towards trimethylamine

 

Reduction

Re-oxidation

Intermediate

K d ,NADPH

k red

k ox

k formation

k decay

(μM)

(s −1 )

(s −1 )

(s −1 )

(s −1 )

Wild type

0.3

1.33

R 1  = 0.79 R 2 = 0.0009

1.580

0.001

N61S

51.8

ND

0.70

0.332

0.048

  

2.42#

   
  1. ND: not detected.
  2. #10-fold higher concentration of NADPH.
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