Figure 2 | Scientific Reports

Figure 2

From: The Effects of Transcription Directions of Transgenes and the gypsy Insulators on the Transcript Levels of Transgenes in Transgenic Arabidopsis

Figure 2

Correlation of RNA blot data and quantitative PCR data. (a) RNA blot analysis of nine independent AVP1/PP2A-C5 co-expressing plants. WT, wild-type plant; A3 to A22, nine randomly selected independent AVP1/PP2A-C5 co-expressing plants. The probes used are indicated on the left. The gene Actin2 was used as RNA loading control. The blots are cropped and the full-length blots are presented in Supplementary Fig. S4. (b) Quantitative PCR analysis of the nine randomly selected AVP1/PP2A-C5 co-expressing plants. Each dot represents an independent transgenic line. The transcript levels of PP2A-C5 and AVP1 relative to the level of Actin2 in wild-type plant were set as 1. The fold of change for AVP1 or PP2A-C5 transcript was used to reflect the overexpression levels of transgenes in transgenic plants. The y-axis represents the fold of change for PP2A-C5 transcript. The x-axis represents the fold of change for AVP1 transcript. Each data point is the mean value of three repeats in RT-qPCR. Error bars represent standard deviations.

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