Figure 1
From: Ilex latifolia Thunb protects mice from HFD-induced body weight gain
Adipose differentiation in OP9 mouse stromal cells. (a) The cells were stained with oil red O at day 5. (b) The absorbance was measured at 510 nm. (c–f) qPCR was used to determine mRNA levels in OP9 mouse stromal cells. The aqueous extract of Ilex latifolia Thunb was added to the medium at 4 μg/mL. PBS was used as the vehicle control. Pparg: Ppar γ ; Ctl: control; R: rosiglitazone; KD: aqueous extract of Ilex latifolia Thunb. β-actin was used as an internal control. Data are presented as the mean ± SEM. Significant differences between Ctl and R are indicated as ##P < 0.01, ###P < 0.001; significant difference versus R + KD or KD are indicated as ***P < 0.001.
