Figure 4
From: Metabolic Profiling of healthy and cancerous tissues in 2D and 3D
Effects of 5FU on 3D spheroid metabolic phenotype versus 2D monolayer in HCT116 colorectal cancer cell line. (A) Basal OCR as a percent of control (untreated 2D or 3D) upon treatment with 2 or 20 mM 5-Fluorouracil in 2D or 3D cell culture model. (B) Basal ECAR as a percent of control (untreated 2D or 3D) upon treatment with 2 or 20 mM 5-Fluorouracil in 2D or 3D cell culture model. (C) Group Key for Panel A,B,D,E. (D) OCR:PPR ratio of 2D or 3D cell model with 5FU treatment (2 or 20 mM). (E) Percentage viability of cells over time in the 2D or 3D model upon exposure to 2 or 20 mM 5FU using Cell Titer Glo assay. (F) A549 spheroid incubated with fluorescent dextran after washing. (G) A549 spheroid treated with fluorescent dextran and 5 nM paclitaxel (pseudo-color yellow).
