Figure 3

Effects of CAR on the morphology and viability of the CSC. U87MG-derived, U343MG-derived and T98G-derived CSCs were treated with complete NSC medium containing DMSO (CTRL) or the indicated concentrations of CAR (100 nM-60 µM) for 3 or 7 days. (A) Representative images were captured after 7 days of incubation, and the area of the culture plates occupied by the spheres (B) was scored using the ImageJ program. The data are presented as the mean values from three independent experiments. For each experimental condition, five pictures were analyzed. U87MG-derived CSCs were treated as described above for 3 days (C) or 7 days (D). Then, cell proliferation was measured using the MTS assay. (E) U343MG-derived and T98G-derived CSCs were treated as described above for 7 days. Then, cell proliferation was measured using the MTS assay. The data are presented as the mean values from three independent experiments, each performed in duplicate. The significance of the differences was determined by one-way ANOVA, followed by Bonferroni’s post hoc test: *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001 vs. the CTRL.