Figure 4

CAR sensitize CSCs to TMZ treatment. U87MG-derived CSCs were treated with complete NSC medium containing DMSO (CTRL) or the indicated concentrations of CAR (100 nM–60 µM) for 7 days. (A) The drug-containing media were replaced with fresh drug-free NSC media and the cells were cultured for an additional 7 days. Then, cell proliferation was measured using the MTS assay. (B) U87MG-derived CSCs were treated in NSC medium with CAR (1–20 µM) and/or TMZ (100–250 µM) for 7 days. Then, cell proliferation was measured using the MTS assay. The data are presented as the mean values from three independent experiments, each performed in duplicate. The significance of the differences was determined by one-way ANOVA, followed by Bonferroni’s post hoc test: *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001 vs. the CTRL.