Figure 5 | Scientific Reports

Figure 5

From: Carnosol controls the human glioblastoma stemness features through the epithelial-mesenchymal transition modulation and the induction of cancer stem cell apoptosis

Figure 5

CAR effects on the cell cycle progression and apoptosis induction of U87MG-CSCs. U87MG-derived CSCs were treated with DMSO (CTRL) or CAR for 3 or 7 days. (A,B) At the end of the treatments, the cell cycle was analyzed. The subpopulations of cells in the different phases of the cell cycle are shown. The data are presented as the mean values from three different experiments. (C–E) Cells were collected and the amount of phosphatidylserine externalization was evaluated using the Annexin V staining protocol. The distribution of the live, early and late apoptotic cells. (E) Representative plots are presented. The data are presented as the mean values from three different experiments. The significance of the differences was determined by one-way ANOVA, followed by Bonferroni’s post hoc test: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. the CTRL; #P ≤ 0.05, ##P ≤ 0.01, ###P ≤ 0.001 vs. CTRL early apoptotic cells.

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