Figure 2 | Scientific Reports

Figure 2

From: The Mammalian Target of Rapamycin and DNA methyltransferase 1 axis mediates vascular endothelial dysfunction in response to disturbed flow

Figure 2

DNMT1 expression and global DNA methylation are elevated in the atherosclerotic endothelium in mice model. (a,b) Expressions of DNMT1, endothelial marker CD31 and methylated 5-cytosine (5-meC) were measured by immunofluorescent staining in aortic outflow from C57BL/6 WT mice with Chow diet (upper panels, yellow arrow heads indicate ECs without DNMT1 or 5-meC expressions) and Apolipoprotein E-deficient (ApoE−/−) mice fed a Western diet (lower panels, white arrow heads indicate ECs expressing high level of DNMT1 expression or 5-meC). (c,d) Expressions of DNMT1, endothelial marker CD31, and methylated 5-cytosine (5-meC) were measured by immunofluorescent staining in the unligated carotid arteries (left panels) and the ligated carotid arteries (right panels) from ApoE−/− mice fed a Western diet. L: lumen; 1 M: one month; 3 M: three months. Images are representative of 8–10 mice with similar results.

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