Figure 3 | Scientific Reports

Figure 3

From: The Mammalian Target of Rapamycin and DNA methyltransferase 1 axis mediates vascular endothelial dysfunction in response to disturbed flow

Figure 3

Intraluminal inhibition of DNMT1 ameliorates endothelial dysfunction and atherosclerosis. (a) Schematic diagram of local delivery of virus. (b) At one week post-surgery, the DNMT1-knockdown efficiency in the ligated carotid arteries was validated by immunofluorescent staining. CD31 indicates the intact endothelia. (c) Cell proliferation marker PCNA (left panels) and inflammatory markers VCAM1 and ICAM1 (middle and right panels) were assessed by immunofluorescence staining in the unligated and ligated carotid arteries subjected to a locally intraluminal incubation with adenovirus expressing shRNA targeting murine DNMT1 (shDNMT1) or control virus. (CL). EL: elastic lamina. (d,e) At four weeks post-surgery, neointimal formation and atherosclerosis were analyzed by haematoxylin and eosin (H&E) and Oil red O stainings in the ligated carotid arteries from ApoE-deficient mice fed a Western diet. The mouse carotid arteries were subjected to a locally intraluminal incubation with ad-mshDNMT1 or control virus. Images are representative of 8–10 mice with similar results. *P < 0.05 vs. CL.

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