Figure 4
From: Novel KIAA0753 mutations extend the phenotype of skeletal ciliopathies
Histology and immunohistochemistry of the growth plate from normal control and affected fetus (P4). (a,b) Hematoxylin-eosin stained sections of the distal femoral growth plate from (a) normal control and (b) affected fetus, respectively. Zones of the growth plate are shown with black lines on the side. R, resting zone; P, proliferative zone and H, hypertrophic zone. Note the abnormal architecture of the proliferative zone in affected fetus with lack of normal chondrocyte columns; (c,d) GAPDH expression (red) is seen in the growth plate for the normal control and P4; (e–j) immunohistochemistry of the proliferative zone from paraffin embedded tissue. KIAA0753 expression (in red) is found in the normal control (e,f) but not in P4 (h,i); (f) and (i) the same area counterstained with DAPI for nuclear staining; (g,j) negative controls for the samples without primary antibody. Scale bar: 100 µm (a–d) and 50 µm(e–j).
