Table 2 Procedural details for standard FISH and RaFISH.
Protocol | Standard | RaFISH |
|---|---|---|
Dewaxing, activation, dehydration | 1 h | 1 h |
Denaturation and hybridization | 16–18 h (37 °C) | 3 h (37 °C), AC |
Washing slides | 10 min | 10 min |
Staining with DAPI | 10 min | 10 min |
Total require time | 18–20 h | 4.5 h |
