Figure 2
Noscapine pre-sensitization followed by docetaxel treatment inhibits growth of mammospheres derived from drug-resistant TNBC cells. (A) Drug-resistant MDA-MB-231 triple negative breast cancer cells were grown as mammospheres as detailed in methods. The mammosphere cultures were either untreated (control) or treated with Noscapine and docetaxel for noted dose and time. The representative microscopic images of mammospheres were showed in Fig. 2A. (B) Number of mammospheres of drug-resistant TNBC cells in each treatment group (control, Noscapine alone DTX alone, noscapine presensitization followed by docetaxel) was quantified microscopically. (C) The cell viabilities of both wild type and drug-resistant TNBC cells were determined by alamar blue-based assay as shown in Fig. 2C, and plotted relative to the values for the respective untreated controls. The histogram columns in panels 2B and 2 C represent means of three independent experiments, respectively. *Indicates a significant (**p < 0.01) difference compared with control. Scale bar 400x. Error bars represents ± SD.
