Figure 7

(A) Brightfield image focused on non-treated SKOV3ip cells of the MCA correlates well with (B) CARS image of −2930 cm⁻¹ peak area. The comparison of (C) the maximum intensity CARS spectrum and average spectrum of the map show similar chemical composition as non-treated OvCa429 samples. (D) The CARS intensity (black) and nonlinear peak fit (orange) is composed of a nonresonant background (green) and two resonant contributions from CH₂ and CH₃ bands at −2850 cm⁻¹ (red) and −2930 cm⁻¹ (blue), respectively. (E) Two spectral clusters with 95% confidence ellipses are discernible in the frequency scatter plot corresponding to lipids (red) and proteins (blue) and φ is the separation angle describing the spectral separation of methylene and methyl groups. (F) The reconstructed map shows the chemical distribution of the segmented lipid (red) and protein (blue) pixels. Experimental parameters: Pump = 720 nm, 25.20 mW; Supercontinuum = 785–945 nm, 3.80 mW; Acquisition time = 500 ms/pixel; Map size = 51 × 51 pixels; Steps = 300 nm; Objective = 100x, 0.9 NA