Figure 4 | Scientific Reports

Figure 4

From: Induction of glucose uptake in skeletal muscle by central leptin is mediated by muscle β2-adrenergic receptor but not by AMPK

Figure 4

Leptin increases glucose uptake and enhances insulin signalling in skeletal muscle of DN-AMPK mice. (a) Representative immunoblot analysis of phosphorylated (pAMPKα) and total (AMPKα1 and AMPKα2) forms of the α1 and α2 subunits of AMPK as well as of phosphorylated and total ACC in soleus muscle of WT and DN-AMPK mice at 6 h after injection of saline (−) or leptin (+) into the VMH. (b) Rate constant of 2DG uptake in soleus, Gastro-R, and EDL muscles at 6 h after injection of saline or leptin into the VMH of WT or DN-AMPK mice (n = 5). (c, d) Representative immunoblot analysis and quantitation (n = 4) of pIR/IR (c) and pAkt/Akt (d) in soleus muscle of WT or DN-AMPK mice at 6 h after injection of saline (−) or leptin (+) into the VMH. Representative immunoblots for α-tubulin were also shown in. (d) All quantitative data are means ± S.E.M. *P < 0.05 versus corresponding value for saline injection in WT mice. ‡ P < 0.05 versus corresponding value for saline injection in DN-AMPK mice (one-way ANOVA and Bonferroni’s multiple-range test).

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