Figure 4
From: Methylomic and phenotypic analysis of the ModH5 phasevarion of Helicobacter pylori
ModH5 ON/OFF state differentially modulates H. pylori P12 flagella number. (a) Paraformaldehyde-fixed, broth-cultured H. pylori modH5 ON (P12 wt), modH5 OFF and ΔmodH5 strains were adsorbed onto formvar copper grids, negative stained with ammonium molybdate and imaged by transmission electron microscopy; 3 representative images shown of each strain (scale bar = 1 μm). Individual bacterial cells were assessed using Fiji ImageJ software for (b) number of flagella per cell (magnification 8000x), (c) flagella length (magnification 8000x), and (d) flagella width (magnification 50,000x). P-values were determined by Kruskal-Wallis test with Dunn’s multiple comparison post-test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; all other comparisons not significantly different. Sample sizes for each group shown in parentheses.
