Figure 2
From: Rapid Purification of Human Bispecific Antibodies via Selective Modulation of Protein A Binding
Mutations disrupt Z-domain interaction. IgG1 variants were eluted from a column comprised of Z-domain using a 30 mL gradient from pH 7.2 to pH 3.0 (dotted line). Variants are indicated on the charts. Absorbance at 280 nm was monitored during elution (solid lines).
