Figure 4 | Scientific Reports

Figure 4

From: Phagocytosis of environmental or metabolic crystalline particles induces cytotoxicity by triggering necroptosis across a broad range of particle size and shape

Figure 4

MLKL deficiency inhibits crystal cytotoxicity in murine primary tubular epithelial cells. Murine primary tubular epithelial cells (pTECs) were isolated from wild-type and Mlkl-deficient mice and exposed to CaP (1 mg/ml), silica (1 mg/ml), TiO2 (0.5 mg/ml), cholesterol (3 mg/ml), CaOx (1 mg/ml), and MSU (0.5 mg/ml) for 24 hrs. (A) Cell death was visualized by PI stain (red color) and Acridine orange (green color). PI images were converted into black and white image for better visualization using ImageJ software. (B) Quantification of DNA-PI mean fluorescence intensity (MFI). Data are expressed as mean ± SEM from three independent experiments.

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