Figure 3 | Scientific Reports

Figure 3

From: The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton

Figure 3

CORO2B localizes to foot processes, the ventral actin cytoskeleton and focal adhesions. (a,b) CORO2B immunogold labeling of glomerular TEM sections shows a prominent localization to the podocyte cell corpus, primary and secondary processes. (c) Immunofluorescence staining for F-ACTIN, VINCULIN and CORO2B-GFP in human immortalized podocytes reveals localization of CORO2B to ventral actin stress fibers and ventral focal adhesions. (d,e) TIRF life cell imaging for CORO2B-GFP and mCherry-PAXILLIN in human immortalized podocytes confirms pronounced localization of CORO2B to the ventral stress fiber - focal adhesions complex and shows focal adhesion recruitment of CORO2B at the late focal adhesion maturation phase.

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