Figure 6 | Scientific Reports

Figure 6

From: The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton

Figure 6

Coro2b knockout influence focal adhesion dynamics. (a–f) Isolated MEF from Coro2b knockout and wild type mice show increased average focal adhesion size but normal focal adhesion numbers in KO cells. Re-expression of CORO2B rescued average focal adhesion size compared to control cells. Immunofluorescence staining visualizes F-ACTIN (Phalloidin) or GFP and the focal adhesion component PAXILLIN (n = 40 WT, 43 KO, 33 WT + GFP, 31 KO + CORO cells out of 3 independent experiments were analyzed; n.s. – non significant, ***p < 0.001, ****p < 0.0001; one way ANOVA and Tukey’s multiple comparisons test was applied). (g–h) Knockout of Coro2b reduces migratory capability of podocytes in single cell migration assays (n = 252 WT and 237 KO cells out of 3 independent experiments were analyzed; ****p < 0.0001).

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