Figure 4
From: The effects of artocarpin on wound healing: in vitro and in vivo studies
ARTO enhances human fibroblast proliferation and migration through the P38 or JNK signaling pathway. (a) Immunohistochemistry was performed to identify vimentin and PCNA (arrows) in wounds on day 15. The cells were pre-treated with MK2006 (MK), PD98059 (PD), SB203580 (SB), or SP600125 (SP) for 1 h and then incubated with ARTO for 24 h. Crystal violet staining (b) and a BrdU incorporation assays (c) were used to measure GM05386 cell number and proliferation. (d) Cell migration was examined via wound healing assays, in which GM05386 cells were wounded by scratch injury (black lines). The wound closure and migration rates were determined. The levels of Akt (e), ERK (f), P38 (g), and JNK (h) were determined by western blot analysis. (i) There was co-localization between vimentin and phosphorylated P38 or JNK in the wounds on day 15. The data are shown as the means ± S.D. N = 3–6 group and *P < 0.05.
