Figure 3
Nucleotidic variation in the CAP-G gene associated with B chromosome presence in E. plorans from Torrox population. The upper panel (white background) shows gDNA counts for 8 nucleotidic positions in 0B and 4B males from Torrox. Note that the 0B reference is usually at lower frequency because of the presence of several gene copies in the B chromosome. The middle panel (grey background) shows RNA counts for these same 8 nucleotidic positions in 0B and 1B females from Torrox. Note that the 0B female has the same nucleotides as the 0B gDNA from Torrox, and that the 1B female has esentially the same nucleotide composition as the B-carrying gDNA libraries, indicating the expression of B chromosome gene copies. The lower panel shows codon (light yellow background) and aminoacidic (yellow background) changes provoked by the 8 substitutions. Note that only nucleotidic changes in positions 643 and 3,010 provoke alterations on the predicted CDS of the B chromosome gene copies (R for C in the 215 residue and E for stop codon in the 1004 residue, respectively), and that the substitution of nucleotide 3,025 is beyond the stop codon.
