Figure 6
WCE modulates myeloid cell activity. (a) The cytokine/chemokine levels of stromal cells in PC-3 and DU145 tumor tissues were analyzed using mouse-specific primers and qRT-PCR. (b) Tumor-derived MDSCs were treated with the indicated compound (10 μmol/L) for 6 h, and proinflammatory genes were analyzed by qRT-PCR. (c) Phosphorylated Stat3 was determined in ex vivo culture of PC-3 tumor-derived MDSCs by FACS following indicated treatment at 10 μmol/L for 6 h. Right, quantitative mean values of triplicate. (d) M1 markers, arginase 1(Arg1) and Ccl2, were analyzed in qRT-PCR under indicated treatments of PC-3 CM or hypoxia (1% O2) plus WCE treatment or vehicle for 12 h. Data are expressed as means ± SEM. **P < 0.01, ***P < 0.001 by unpaired Student’s t-test.
