Figure 6

Glycogen storage is required for the cellular immune response against wasp infection. (A) Glycogen contents in skeletal muscles, normalized to protein, when suppressing JAK/STAT signaling and insulin signaling respectively in muscles with the indicated genetic constructs. (B) Glycogen contents in skeletal muscles normalized to protein, at different times after wasp infection. (C) Glycogen contents in skeletal muscles normalized to protein when suppressing glycogen synthase or glycogen phosphorylase in muscles by UAS-GlyS ^RNAi or UAS-GlyP ^RNAi, respectively, with the Mef2-GAL4 driver. (D) Encapsulation rates when suppressing glycogen synthase or glycogen phosphorylase in muscles by UAS-GlyS ^RNAi or UAS-GlyP ^RNAi, respectively, with the Mef2-GAL4 driver. (E,F) Number of lamellocytes (E) and plasmatocytes (F) per larva after 12 h wasp infection when suppressing glycogen synthase in muscles by UAS-GlyS ^RNAi, with the Mef2-GAL4 driver. (G) Feeding activity when depleting glycogen in muscles by expressing UAS-GlyS ^RNAi with the Mef2-GAL4 driver. (H) Encapsulation rates under starvation conditions. (I,J) Number of lamellocytes (I) and plasmatocytes (J) per larva after 12 h wasp infection under starvation conditions. Data information: Encapsulation rates were determined in at least three independent experiments, and in total at least 100 larvae were analyzed. For hemocyte counts, at least eight larvae were analyzed for each genotype. For glycogen measurements, at least three independent experiments were done. Bars show averages and standard deviations. The P-values (unpaired t-test, unequal variance) are indicated, NS: not significant.