Figure 2
More representative metabolites for each cell type and the related biological pathways. (A) Pareto analysis of main retrieved metabolites’ concentration for each cell type. The histograms report the bars in decreasing order for each cell type. The curve on the plot accounts for the cumulative percentage. The corresponding values are reported on the secondary y-axis. (B) The analysis of more nine representative metabolites in each cell type reveals that both common (Lac, Ace) and differential metabolites (Tau and Glc for NSCs; Ala for OECs) comprise for more than 74% of total metabolome. A search in the Kegg pathway database reveals main biological topics involving the different metabolites: all NSCs showed higher Tau and Glc levels whereas larger Lac amounts characterized OECs. More details on all the involved biological pathways analyzed are reported in Supplementary Fig. S5. Abbreviations: ABC transporters = ATP-binding cassette transporters; Aromatic a.a. = aromatic amino acids (Tyrosine, Triptophan, Phenylalanine); PPAR = Peroxisome proliferator-activated receptors; HIF-1 = Hypoxia-inducible factor 1; FoxO = Forkhead box O transcription factors; SC = Stem Cells; Sulfur containing a.a. = Sulfur containing amino acids.
