Figure 1

Classification of the immunoisolated, stable CCV from σ1B −/− and wt synapses by comparing their coat protein composition. Data are presented as box-plot diagrams and show the quantification of independently prepared biological samples (wt = 100%). Numbers given below the box plots indicate the independently performed experiments. (A) Analysis of coat proteins in stabilised CCV are shown as blue boxes. The comparison of the dynamin content in the total synaptic CCV pool is shown as a white box. (B) Representative semi-quantitative western-blot images used in the quantification shown in A (please also refer to Mat. & Meth.). (C) Comparison of the association of the clathrin basket disassembling Hsc70 and its two co-chaperones with the immunoisolated, stable CCV from σ1B −/− and wt synapses. (D) Representative images of the semi-quantitative western-blots analysis used for the quantification are shown in C (please also refer to Mat. & Meth.).