Figure 3

(A) Comparison of the expression and the distribution of synaptojanin-1 and its splice variant in σ1B −/− and wt cortices and of their association with the total pool of synaptic CCV (Σ CCV) and with the immunoisolated, stable CCV (st CCV, blue boxes) from σ1B −/− and wt synapses. (B) Representative western-blots of the analysis shown in A. (C) Comparison of the expression and the distribution of endophilin A1 in σ1B −/− and wt cortices and of its association with the total pool of synaptic CCV and with the stable CCV from σ1B −/− and wt synapses. (D) Association of amphiphysin with the total pool of synaptic CCV and with the stable CCV from σ1B −/− and wt synapses. (E) Representative western-blots of the analysis shown in (C and D). Box-plot diagrams show the quantification of semi-quantitative western-blots of independently prepared biological samples (wt = 100%). Numbers given below the box plots indicate the independently performed experiments. (F) Comparison of the association of the co-adaptor proteins AP180 and NECAP-1 with the stable CCV (st CCV, blue box) and synaptic CCV (Σ CCV, white box) from σ1B −/− and wt synapses. Quantification of semi-quantitative western-blots of independently prepared biological samples (wt = 100%). Numbers given below the box plots indicate the independently performed experiments. (G) Representative western-blots of the analysis shown in A.