Figure 4

(A) Comparison of the association of the co-adaptor protein stonin 2 with stable CCV (st CCV, blue) isolated from σ1B −/− and wt synapses. (B) Comparison of the expression of the active zone protein Git1 and in (C) of Arf6 in σ1B −/− and wt synapses and of its association with the total pool of synaptic CCV (Σ CCV) and with the stable CCV (st CCV, blue box) from σ1B −/− and wt synapses. Quantification of semi-quantitative western-blot signals of independently prepared biological samples (wt = 100%). Numbers given below the box plots indicate the independently performed experiments. (D) Representative western-blot images used for the analysis shown in A and B. (E) Comparison of the endocytosis of the active zone Munc13 proteins by AP-2 CCV (Σ CCV) in σ1B −/− and wt synapses. Quantification of semi-quantitative western-blots of independently prepared biological samples (wt = 100%). Numbers given below the box plots indicate the independently performed experiments. (F) Representative western-blots of the analysis shown in A. (G) Comparison of the SV protein content between stable synaptic CCV isolated from σ1B −/− and wt synapses as determined by quantitative mass-spectrometry (iTRAQ). Proteins with significantly different distributions are indicated by black dots, unaltered proteins with gray dots. VAT-proteins indicate subunits of the SV V-ATPase.