Figure 2 | Scientific Reports

Figure 2

From: Elevation in tropical sky islands as the common driver in structuring genes and communities of freshwater organisms

Figure 2

Spatial genetic structure for four Ephemeroptera species showing wide ecological distributions, using restriction-site-associated-DNA sequencing (RADseq). Pie-charts on the map for each species represent the proportion of samples assigned to each cluster within each sampling location. Spatial structure was computed using STRUCTURE, by examining K values ranging from one to eight, and replicating analyses five times. A threshold of 0.95 in the assignment probability was applied to assign samples to a given cluster. The optimal number of genetic clusters was established by inspection of the likelihood function. Visual examination of the distribution of each cluster indicates that populations from a similar elevation tend to be characterized by the same genetic cluster (see also, Supporting Information S3). Maps were generated with the Quantum GIS geographic information system (QGIS 2.18; QGIS Development Team, 2016. QGIS Geographic Information System. Open Source Geospatial Foundation Project; available at https://www.qgis.org).

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