Figure 7 | Scientific Reports

Figure 7

From: Functional aspects of salivary nitric oxide synthase of Rhodnius prolixus (Hemiptera, Reduviidae) and nitric oxide trafficking at the vector-host interface

Figure 7

Alterations in the diameter of the mouse ear skin venules throughout the time period after tail-vein intravenous microinjections with R. prolixus salivary gland extracts (SGEs), which were obtained by disruption of three salivary gland (SG) pairs in a PBS-pH 7.4 solution or in the presence of bovine serum albumin (BSA-pH 7.4 solution) (A), or using R. prolixus SG extracts disrupted in a BSA-pH 7.4 solution from insects injected with 5 µg keratin dsRNA (dsKer), nitric oxide synthase dsRNA (dsNOS) or nitrophorins dsRNA (dsNPs). The venule diameter before the injection was arbitrarily set to 1 to allow a relative comparison. On the X axis, -1 corresponds to the moment before the SGE injection, and 0 corresponds to the moment immediately after the SGE injection (black arrows). Data are represented by the mean ± standard error (n = 5). Statistical analysis was performed using two-way repeated measures ANOVA, *Bonferroni’s test, p < 0.05, **Bonferroni’s test, p < 0.01.

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