Figure 8 | Scientific Reports

Figure 8

From: Functional aspects of salivary nitric oxide synthase of Rhodnius prolixus (Hemiptera, Reduviidae) and nitric oxide trafficking at the vector-host interface

Figure 8

Alterations in the diameter of the mouse ear skin venule over time after the ear intradermal microinjections of 32.2 nL of R. prolixus salivary gland extracts (SGEs), which were obtained by (A) salivary gland (SG) disruption in PBS-pH 7.4 solution or disruption in the presence of albumin (BSA-pH 7.4 solution). (B) Activity levels of the SGEs from the R. prolixus injected with keratin dsRNA (dsKer), nitrophorins dsRNA (dsNPs) or nitric oxide synthase dsRNA (dsNOS) that were disrupted in the BSA-pH 7.4 solution. In the negative control, only the BSA-pH 7.4 solution (without the SGE) was injected. The values of maximum dilation were arbitrarily set to 1 to allow a relative comparison in each experiment. On the X axis, -1 corresponds to the moment before the SGE injection, and 0 corresponds to the moment immediately after the SGE injection (black arrows). Data are represented by the mean ± standard error (n = 5 for each time point). The analysis was performed using two-way repeated measures ANOVA and Bonferroni’s test, *p < 0.05, **p < 0.01.

Back to article page