Figure 6

(A) Neuronal differentiation after protocol 1 of BrdU administration. (B) Number of BrdU⁺ cells and (C) new neurons (BrdU⁺/NeuN⁺ cells) in the ipsilesional cortex from 7-28d after MCAO. (D) Brain image showing the cortical regions analyzed and representative images of a BrdU⁺/NeuN⁺/TO-PRO-3⁺ cell (scale bar: 25 µm). Sham mice represented in Fig. 6B and C were sacrificed at 7 days, and did not show any differences with 14d and 28d sham. Data are expressed as mean +/− SEM (n = 5–8). Non-parametric 1-way ANOVA followed by Dunnett post-hoc testing were used to analyze data (BrdU⁺: *p = 0.0421 MCAO vs. Sham; BrdU⁺/NeuN⁺: *p = 0.0011 MCAO vs. Sham).