Figure 1

Effects of MAM with roscovitine or db-cAMP on maturation, embryo development and chromatin configuration of prepubertal (graphs A to D) and adult (graphs E and F) mouse oocytes. In graph A, freshly collected (FC) prepubertal oocytes were separated according to chromatin configuration and the resulting SN and NSN DOs were separately matured in 199-2 with cumulus monolayer coculture. In graph B, prepubertal COCs were matured in 199-2 following MAM for 4 h with 100 µM db-cAMP (dbc) or 100 µM roscovitine (Ros). In graph C, prepubertal DOs of NSN configuration were matured in 199-2 with cumulus monolayer following MAM for 4 h with dbc or Ros. Graphs D and E show distribution of different chromatin configurations following MAM of prepubertal and adult COCs, respectively, for 4 h with dbc or Ros. In graph F, adult COCs were blocked for 4 h in 199-1 containing dbc or Ros before in vitro maturation for embryo development. In graphs A,B,C and F, each treatment was repeated 6-7 times and each replicate contained about 35 oocytes. In graphs D and E, each treatment was repeated 4–5 times and each replicate contained about 20 oocytes. MII: % MII/cultured oocytes; Act: % Activated/MII oocytes; 2-C: % 2-cell embryos/activated oocytes; 4-C: % 4-cell embryos/2-C embryos; Blst: % Blastocysts/4-cell embryos. a,b: Values with a different letter above bars differ significantly (P < 0.05) within developmental stages or oocyte treatment groups.