Figure 5
Contents and activity of CDK5 in mouse oocytes. (A to J) Show contents of CDK5 and p35 in NSN or SN oocytes from prepubertal (Prep) or adult mice before or after MAM of DOs with 100 µM db-cAMP (dbc) or roscovitine (Ros). Micrographs A/A’ to H/H’ are laser scanning confocal images. A and A’ etc. indicate the same oocytes observed using lasers and filters of different wave lengths following staining with anti-CDK5 or p35 antibodies (red) and Hoechst 33342 (blue), respectively. Bar is 12 µm and applies to all images. D/D’ and H/H’ show negative control (NC) oocytes processed with primary antibodies omitted. Graphs I and J show relative levels of CDK5 and p35, respectively, in prepubertal NSN or SN and adult SN oocytes before (FC) or after MAM with dbc or Ros. Each treatment was repeated 3 times and each replicate contained 10 oocytes. Graphs K and L show percentages of metaphase II (MII) and activated (Act) oocytes, 2-cell and 4-cell embryos and blastocyst (Blst) and cell number per blastocyst (Cell No) following prepubertal (K) or adult (L) COCs were blocked for 4 h with dbc and 100 (dR100), 50 (dR50), 25 (dR25), 12.5 (dR12.5), 125 (dR125) or 150 (dR150) µM Ros. Each treatment was repeated 3 times with each replicate containing about 30 oocytes. a,b: Values with a different letter above bars differ significantly (P < 0.05). In graph L, percentages of MII and activated oocytes, 2- and 4-cell embryos and blastocysts did not differ between treatments.
