Figure 8

More activated CD4⁺-T cells with a Th₁ and Th₁₇ profile and less regulatory T cells in TAC than sham-operated cMy-mOVA-OT-II mice. (a) The proportion of CD3⁺CD4⁺ T helper cells among splenocytes was determined by flow cytometry 10 weeks after sham or TAC operation of cMy-mOVA and cMy-mOVA-OT-II mice. The proportion of activated CD25⁺ cells (b) and CD69⁺ cells (c) among CD3⁺CD4⁺ T helper cells has been determined in parallel. (d) The proportion of CD25⁺FOXP3⁺ regulatory T cells among CD4⁺-T cells was analyzed in addition. (e) The proportion of CD4⁺-T cells expressing the indicated cytokines was also determined by flow cytometry directly ex vivo without any further stimulation. Means plus SEM are shown and the numbers of mice analyzed are indicated in the panels a to d beside the bars. In panel e, the numbers of analyzed sham and TAC-operated cMy-mOVA and cMy-mOVA-OT-II mice were: 15/8/10/17 for IL-4 and IL-17F; 14/7/10/17 for IL-2, IL-6, IL-10, and IFN-γ; 9/7/10/17 for IL-17A, TNF-α, and TGF-β. Differences between two groups were analyzed by U-tests and significant P-values are indicated (*P < 0.05, **P < 0.01, ***P < 0.001).