Figure 1 | Scientific Reports

Figure 1

From: Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein

Figure 1

Construction of the hFGF21 expression vectors and schematic representation of the domain structure of fusion proteins. (A) The vector map of pHMGWA-hFGF21 for the Gateway® cloning method. Gene expression of the fusion proteins is under control of the IPTG-inducible T7 promoter. Ampicillin served as a selection marker. (B) Schematic structure of the eight tagged proteins His6-, Sumo-, Trx-, GST-, PDIb′a′-, MBP-, NusA-, and PDI-hFGF21 (total size). The arrow indicates the TEV protease cleavage site between the tags and hFGF21.

Back to article page