Table 1 Expression and solubility levels of hFGF21 fused with eight different tags.
Tag | Tag size (kDa) | Fusion protein size (kDa) | Expression level (%) | Solubility (%) | ||
|---|---|---|---|---|---|---|
37 o C | 18 o C | 37 o C | 18 o C | |||
His6 | 0.8 | 24.1 | 25.8 | 29.1 | 11.5 | 36.2 |
Sumo | 11.3 | 35.8 | 29.4 | 31.8 | 48.8 | 63.5 |
Trx | 11.7 | 35.9 | 29.2 | 36.4 | 12.4 | 48.0 |
GST | 25.6 | 49.8 | 17.8 | 10.6 | 15.6 | 62.2 |
PDIb′a′ | 30.6 | 55.1 | 29.7 | 29.8 | 23.1 | 85.1 |
MBP | 40.2 | 64.4 | 35.9 | 40.6 | 60.5 | 88.6 |
NusA | 54.7 | 79.0 | 37.7 | 43.5 | 23.2 | 89.1 |
PDI | 54.8 | 79.3 | 13.6 | 15.1 | 82.1 | 88.3 |
