Table 2 Prokaryotic purification of hFGF21 from solubly expressed MBP-FGF21.
Purification step | Total protein (mg) | Purity (%) | hFGF21 (mg) | Yield (%) |
|---|---|---|---|---|
Cell weight | 1900 (pellet) | — | — | |
Supernatant | 252.0 | 40.6 | 30.9 | 100 |
1st IMAC | 50.0 | 73.1 | 11.0 | 34.0 |
2nd IMAC | 8.5 | 95.4 | 8.1 | 26.2 |
