Figure 5

TbKu but not TbMRE11 is necessary for normal levels of 5′ TTAGGG 3′-ending telomere G-overhangs. The adaptor ligation assay was performed using genomic DNA isolated from BF TbMRE11 null⁵⁵ and TbKu80 null cells (a) or from TbKu80 null cells and TbKu80 null cells that also express an ectopic TbKu80-GFP allele (b). The ethidium bromide-stained gels are shown on the left and the exposed images are shown on the right. (c) Quantification of the adaptor ligation results in TbMRE11 null cells, TbKu80 null cells, and TbKu80 null cells that express an ectopic TbKu80-GFP allele in comparison to that in WT cells. Average values are calculated from three (TbMRE11 null), five (TbKu80 null), or three (TbKu80 null + TbKu80-GFP) independent experiments. Error bars represent standard deviation. The overhang signals from TbKu80 null and TbMRE11 null cells were compared to those in WT cells by unpaired two-tailed t tests. **P < 0.01. P values greater than 0.05 are not indicated.